Sandhya Mukherjee was born on 4th of October 1931 in Calcutta, India. After her matriculation she started learning classical music under Shri Santosh Basu. Later on she took her training under Shri Gemini Ganguly, Prof. A Kannan and Prof. Chinmaya Lahiri. In 1950 she became a disciple of Ustad Bade Ghulam Ali Khan followed by his son Ustad Munavvar Ali Khan.
R C Boral, the music director of New Theaters gave her a song "Ha Ha Ha Hans Ke Jiye Ja" in a film called "Anjaan Garh". She later sang "Ashkon Me Chhipi Mohabbat Ki Kahani" in Pehla Aadmi, which gave her immense popularity in Calcutta. Then she moved to Bombay where she earned fame with a duet with Hemant Kumar "Aa Gupchup Gupchup Pyar Karen" in Sazaa. Later, she sang for Anil Biswas in "Taraana" a duet with Lata "Bol Papiha Bol Re" which was an instant hit. She got films like Ratnadeep, Husn Ka Chor, Fareb etc, but she could not sing for a long time because of her training in classical, her voice was best suited for that.
Her voice had the same Bengali touch as that of Geeta Dutt. In 1966, she married poet Shyamal Gupta and settled with her husband in Calcutta.